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Medical laboratory equipment ORF1ab E N gene detection kit fluorescent PCR method

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  • Real-time PCR Detection Kit | Morel Medical

    The kit is a TaqMan probe real-time fluorescent PCR to qualitatively detect ORF1ab, N and E genes nucleic acid of 2019-nCoV in throat swab, sputum in patients with respiratory infection symptoms and close contacts.The test results are for clinical reference only and cannot be used as a basis for confirming or eliminating cases. Operators should have experience of professional training in gene ...

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  • Xiamen Zeesan Biotech Co., Ltd. of Xiamen Fujian at

    2021-4-20 · This kit is used for in vitro qualitative detection of novel coronavirus (SARS-CoV-2) ORF1ab and N gene in samples, including nasopharyngeal swab, oropharyngeal swab, bronchoalveolar lavage fluid and stool, for diagnosis of the COVID-19 infection, the suspected cluster cases and of the patients who need differential diagnosis of COVID-19 infection.

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  • (Convergys® PCRtist real time fluorescent PCR method)

    2020-2-27 · Convergys® COVID-19 RNA Detection Kit (Convergys® PCRtist real-time fluorescent PCR method) User Manual Version: V-1.0, Feb., 2020 Product name: Convergys® COVID-19 RNA Detection Kit Packing specifications: 24 tests /kit Intended use: The kit is used to qualitatively detect the ORF1ab gene and N gene of the new

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  • New Coronavirus (2019-nCoV) Nucleic Acid Detection

    The PerkinElmer New Coronavirus Nucleic Acid Detection Kit is a real-time RT-PCR test intended for the qualitative detection of nucleic acid from SARS-CoV-2 in human oropharyngeal, nasopharyngeal, and anterior nasal swab samples. ... (N) gene and ORF1ab. Thus mutations in spike protein, as seen in the B.1.1.7 and B.1.351 lineages, do not impact ...

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  • Molecular diagnosis of COVID‑19: Current situation

    The Department of Clinical Laboratory of the Third Hospital of Chongqing Municipal People's Hospital compared the detection performance of 2019-nCoV for six of the kits (Shengxiang Biotechnology 2019-nCOV rapid nucleic detect kit, Beijing Ka You Di 2019-nCoV ORF1ab/N gene RNA detection kit for nucleic acid-free extraction, Shuoshi Biotechnology 2019-nCOV detection kit, Zhongyuan 2019-nCOV ...

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  • COVID-19 Diagnostics In Context For updated version see ...

    2020-12-22 · COVID-19 Diagnostics In Context CSB Nucleic acid tests (NATs, for viral RNA) Most common targets: Viral genome sequence #MN908947 , • CDC approved targets: N1, N2 genes (single or multiple) • Other emerging targets: E gene, S gene, Orf1ab gene, RdRP gene Key reagents: CDC approved kits include 2019-nCoV CDC Probe and Primer Kit for SAR2-CoV-2 (Biosearch Technologies) and 2019-nCoV Kit …

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  • RT‐LAMP for rapid diagnosis of coronavirus

    Medical Laboratory of Shenzhen Luohu People's Hospital, Shenzhen, 518001 China ... assay are #1, #2, #3 in order from left to right. Tube #1 and #2 separately contain O117 and N15 primers targeting the Orf1ab gene and N gene of SARS‐CoV‐2. Tube #3 contain human β‐actin primers. ... A commercial 2019‐nCoV RT‐PCR kit (Shanghai ZJ Bio ...

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  • COVID-19 Coronavirus Real Time PCR Kit

    COVID-19 Coronavirus Real Time PCR Kit is an In Vitro Diagnostic (IVD) reagent replying on fluorescent PCR technology and aiming at qualitatively detect SARS-CoV-2 from upper and lower respiratory tract specimens. Upper respiratory tract specimens include throat swab and nasopharyngeal swab. Lower respiratory tract specimens include sputum.

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  • Top 100 Coronavirus Testing Kit Manufacturers and

    2021-4-21 · Their LiliF™ COVID-19 Real-time RT-PCR Kit can detect the novel coronavirus using probe method of Real-time RT-PCR, through the reacting of the specific primer and Fluorescent probe in sample. KH Medical Co. Ltd. KH Medical is Manufacturer and Supplier of Diagnostic Products founded in Seoul, South Korea. The company recently announced that ...

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  • SARS-CoV-2 diagnostic pipeline - FIND

    FIND is collating an overview of SARS-CoV-2 tests that are commercially available or in development for the diagnosis of COVID-19. For immunoassays and molecular tests, we have compiled a fully searchable directory.Manufacturer performance data (sensitivity and specificity) are included, where known, alongside data from independent evaluations conducted by FIND, where applicable.

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  • Xiamen Zeesan Biotech Co., Ltd. of Xiamen Fujian at

    2021-4-20 · This kit is used for in vitro qualitative detection of novel coronavirus (SARS-CoV-2) ORF1ab and N gene in samples, including nasopharyngeal swab, oropharyngeal swab, bronchoalveolar lavage fluid and stool, for diagnosis of the COVID-19 infection, the suspected cluster cases and of the patients who need differential diagnosis of COVID-19 infection.

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  • China Pcr Kits, China Pcr Kits Manufacturers and

    ORF1ab E N gene test Fluorescent Probe real time PCR kits. Hangzhou Skyfire Biotech Co., Ltd. ... Good performance rt-pcr detection kit real time test pcr kit. ... BS-A200 High Precision Bk-Eo/Bk-Tc 96 Wells*0.2ml Thermal Cycler Standard Medical Equipment Mg108+ Pcr Test Kit.

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  • SARS-COV-2 PLATE-BASED PCR | Research Institute

    2019-Novel Coronavirus (ORF1ab, E & N genes) nucleic acid test kit (Fluorescent Probe-based real-time PCR assay) Jiangsu Mole Bioscience Co. Ltd, Jiangsu Province China: 0.7931: 1: Nov 22 - Dec 21, 2020: 21: 2019-Novel Coronavirus (ORF1ab, E & N genes) nucleic acid test kit (Fluorescent Probe-based real-time PCR assay) - Re-analysis using new IFU

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  • (Convergys® PCRtist real time fluorescent PCR method)

    2020-2-27 · Convergys® COVID-19 RNA Detection Kit (Convergys® PCRtist real-time fluorescent PCR method) User Manual Version: V-1.0, Feb., 2020 Product name: Convergys® COVID-19 RNA Detection Kit Packing specifications: 24 tests /kit Intended use: The kit is used to qualitatively detect the ORF1ab gene and N gene of the new

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  • U-TOP™ COVID-19 Detection Kit

    2021-2-16 · The U-TOPTM COVID-19 Detection Kit uses dual -labeled Peptide Nucleic Acid (PNA) probes that target two distinct regions in ORF1ab and one region in N gene of SARS-CoV-2 genome.

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  • Test Kits to Detect SARS-CoV-2 - rapidmicrobiology

    The Nucleocapsid Protein is the most abundant protein in SARS-CoV-2. The N-protein is a highly immunogenic phosphoprotein and rarely changes. The N protein of SARS-CoV-2 is often used as a marker in diagnostic assays. There is also the hemagglutinin-esterase dimer, a membrane glycoprotein, an envelope protein, and RNA.

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  • (PDF) Molecular and Serological Tests for COVID-19 a ...

    PCR Detection Kit CE-IVD . ... in the regions of ORF1ab, S gene, and N gene. For the interpretation of results, a colorimetric method . ... Real-time Fluorescent RT-PCR kit for .

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  • BGI Local Laboratory Solution for Non-invasive

    BGI’s Real-Time Fluorescent RT-PCR kit for detecting 2019-nCoV(SARS-CoV-2) is a qualitative in vitro nucleic acid amplification assay used to detect SARS-CoV-2 using reverse transcription PCR from throat swab and Bronchoalveolar Lavage Fluid(BALF) samples. The kit is widely used for rapid detection and outbreak control of COVID-19 in China.

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  • (PDF) RT‐LAMP for rapid diagnosis of coronavirus

    LAMP results of four different sets of primers targeting the DNA of N, S and Orf1ab gene of SARS-CoV-2. A. Performance of N-1 primer set using N gene DNA sequence.

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  • Molecular diagnostic technologies for COVID-19 ...

    2020-11-1 · The findings of high-throughput genomic sequencing of SARS-CoV-2 are regularly deposited on the global initiative on sharing all influenza data (GISAID) .The development of oligonucleotides (primers and probes) for molecular diagnosis of COVID-19 was initiated as soon as these findings were made public on 10th Jan 2020 , .CDC (USA), China CDC, Charité Germany, Institut Pasteur (France), …

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  • SARS-COV-2 PLATE-BASED PCR | Research Institute

    2019-Novel Coronavirus (ORF1ab, E & N genes) nucleic acid test kit (Fluorescent Probe-based real-time PCR assay) Jiangsu Mole Bioscience Co. Ltd, Jiangsu Province China: 0.7931: 1: Nov 22 - Dec 21, 2020: 21: 2019-Novel Coronavirus (ORF1ab, E & N genes) nucleic acid test kit (Fluorescent Probe-based real-time PCR assay) - Re-analysis using new IFU

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  • Biologix launched a variety of novel

    一、Novel coronavirus (COVID-19) nucleic acid detection kit Biologix provides specific primers and fluorescent probes designed for COVID-19 (ORF1ab E N gene) to the international market, and uses real-time fluorescent quantitative PCR technology to detect the nucleic acid of new coronavirus

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  • Title page Metabolic disturbances and inflammatory ...

    2020-3-26 · (2019-nCoV) ORF1ab/N gene double nucleic acid detection kit (fluorescent PCR method) was purchased from the Shanghai Jienuo Company. The detection equipment QuantstudioDx and 7500 fluorescent PCR instrument were purchased from ThermoFisher Company of the It is made available under a CC-BY-NC 4.0 International license.

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  • Buy Acid Probe Test in Bulk from China Suppliers

    Test Kit Nucleic Acid Detection Kit-2. Real Time PCR Kit for (ORF1ab, N) INSTRUCTION FOR USE Product Name Real Time PCR Kit Product Description The ... qualitative detection of ORF1ab gene, N gene and E gene, reduce recheck. US 15 Unit. 10000 Units ... the test can be conducted without any other laboratory equipment and skilled peop. US 40 ...

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  • One-tube SARS-CoV-2 detection platform based on

    2021-2-16 · Cycle Threshold (CT) values of N and Orf1ab genes were assessed using the SARS-CoV-2 test kit from BioGerm (Additional file 1: Table S1). We found that 4001 N was a positive sample (FC-RdRp is 1.9 and FC-N is 4.6) by OR-DETECTR, and CT values of 4001 N were 38.9 (Orf1ab) and 36.8 (N gene), meaning this clinical sample had very low viral loads.

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  • COVID-19 Coronavirus PCR Kit, UK Variant| RT-Digital

    Catalog #: 02.01.1020. The 1 step SARS-CoV-2 detection kit is based on the PCR method which uses a fluorescent probe and a specific primer to detect two specific regions within the novel coronavirus (SARS-CoV-2) nucleocapsid protein N gene. This molecular panel aids in the detection of viral RNA from SARS-CoV-2, the causative agent of COVID-19.

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  • COVID-19 Pandemic: Review of ... - Dove Medical Press

    FDA also approved TaqPath COVID-19 Combo kit (ThermoFisher-Applied Biosystems), Smart Detect SARS-CoV-2 rRT-PCR Kit (InBios International, Inc.), Biomeme SARS-CoV-2 Real-Time rRT-PCR Test (Biomeme, Inc) which detect Orf1ab, E, N, and S gene (mentioned in Table 2). Besides several diagnostic kits like US Food and Drug Administration (USFDA ...

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  • Loop‐mediated isothermal amplification (LAMP): An ...

    Most of the studies targeted the conserved sequence of nucleocapsid gene (N gene) and ORF1ab (Figure.4) because of their high homology and their divergence from the other coronaviruses. 29 The N gene is at the 3'‐end of the SARS‐CoV‐2 RNA, 52 ORF1ab encodes the replicase polyprotein and it is about 21‐kb long. 53 Dao Thi et al. 37 ...

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  • PowerPoint Presentation

    detection kit (PCR fluorescent probe method). There was no false negative and the coincidence rate of positive reference products was 6/6. The enterprise negative reference of Influenza viruses were extracted by this product, and the extracted nucleic acid were detected by Influenza A/B nucleic acid detection kit. There was

    Get Price
  • A Novel Approach to the Bioluminescent Detection of

    The COVID-19 pandemic caused by the SARS-CoV-2 virus, which first emerged in December 2019, represents an ongoing global public health emergency. Here, we developed an improved and highly sensitive approach to SARS-CoV-2 detection via coupling bioluminescence in real-time (BART) and reverse-transcriptase loop-mediated amplification (RT-LAMP) protocols (RT-LAMP-BART) and was …

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  • Molecular diagnosis of COVID‑19: Current situation and ...

    ORF1ab/N gene double fluorescent RT‑PCR kit) and reported that the detection capabilities of each kit for weakly positive samples were different (24). Furthermore, certain kits were able to double‑positively detect Orf1ab and N, while other kits could only detect one of them. At present, RT‑PCR nucleic acid detection serves an irre‑

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  • P0180 COVID -19 Real Time PCR Test

    2021-3-4 · By using multiplex PCR-fluorescent probes, combined with fast one-step RT-PCR technology, the kit uses COVID-19 ORF1ab and the specific conserved sequence of nucleocapsid protein N gene as target regions. With the designed dual-target gene detection primer probe, the kit works with a high-efficiency RT-PCR reaction system for detection.

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  • Ultra-rapid Real-time RT-P R Assay for OVI -19

    2020-3-22 · This kit does not need for separate extraction and purification steps of nucleic acid. Patient’s sample can be mixed directly with the real time PCR reagents and tested on the qPCR devices. This kit can also distinguish the differences and similarities of ORF1ab gene and N-gene among various strains of …

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  • One-tube SARS-CoV-2 detection platform based on

    2021-2-16 · Cycle Threshold (CT) values of N and Orf1ab genes were assessed using the SARS-CoV-2 test kit from BioGerm (Additional file 1: Table S1). We found that 4001 N was a positive sample (FC-RdRp is 1.9 and FC-N is 4.6) by OR-DETECTR, and CT values of 4001 N were 38.9 (Orf1ab) and 36.8 (N gene), meaning this clinical sample had very low viral loads.

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  • Anitoa Lab on Chip Product Requirement Specification

    2020-2-28 · the PCR reagents and tested on the PCR devices. This kit can also distinguish the differences and similarities of ORF1ab gene and N-gene among various strains of coronavirus. Specific primer probes are designed for this single-step RT-qPCR kit. A primer probe using human β-Globin gene sequence as a template is used for internal control.

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  • COVID-19 Pandemic: Review of ... - Dove Medical Press

    FDA also approved TaqPath COVID-19 Combo kit (ThermoFisher-Applied Biosystems), Smart Detect SARS-CoV-2 rRT-PCR Kit (InBios International, Inc.), Biomeme SARS-CoV-2 Real-Time rRT-PCR Test (Biomeme, Inc) which detect Orf1ab, E, N, and S gene (mentioned in Table 2). Besides several diagnostic kits like US Food and Drug Administration (USFDA ...

    Get Price
  • buy covid-19 pcr test kits online by Fosun EUA US

    Novel Coronavirus (2019-nCoV) RT-PCR Detection Kit (Commercial names: Fosun 2019-nCoV qPCR) SIZE of Covid-19 Pcr test. 32 tests/kit, 48 tests/kit, 96 tests/kit. how we can use COVID-19 Fousen Pcr Test Kits. This product is intended for the rapid detection of 2019-nCOV in human throat swab or sputum samples. Coronavirus is a +ssRNA virus with an ...

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  • How SARS-CoV-2 Tests Work and What's Next in

    In particular, the test detects the presence of SARS-CoV-2’s E gene, which codes for the envelope that surrounds the viral shell, and the gene for the enzyme RNA-dependent RNA polymerase. Yvonne Doyle, the medical director and the director of health protection for Public Health England, tells The Scientist in an email that once a sample is ...

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  • PowerPoint Presentation

    detection kit (PCR fluorescent probe method). There was no false negative and the coincidence rate of positive reference products was 6/6. The enterprise negative reference of Influenza viruses were extracted by this product, and the extracted nucleic acid were detected by Influenza A/B nucleic acid detection kit. There was

    Get Price
  • Nucleic acid visualization assay for Middle East ...

    2021-3-1 · The World Health Organization (WHO) recommends real-time RT-PCR targeting the sequence upstream of the envelope protein gene (UpE) and the open reading frame 1ab (ORF1ab) or the gene encoding the nucleocapsid protein gene (N) as the screening and diagnostic targets for MERS-CoV [10–12]. Real-time RT-PCR has great advantages in terms of high ...

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